ポッター[‐エルベージェム]ホモジナイザーによる均質化

Potter homogenization is one of the homogenization methods – for “lysing” (breaking apart) cells mechanically. The products (“homogenate”) are centrifuged at different speeds to obtain different content of the cells (e.g. DNA, proteins) according to densities.

Here, the “Potter homogenizer” is being used. (A picture is available in the links below)
http://www.icampus.ucl.ac.be/SBIM2520/document/genemol/first...
What's a Potter homogenizer? It's a device used to grind organs or parts of organs. There are several types of homogenizers. The one you will use is known as a Potter.
We immersed the in Sulfochromic acid, Rinse them with DEPC water to make them RNase free.

http://images.google.com/images?hl=en&lr=&q=Potter-Elvehjem ...

Cross ref. from Glova:
hand homogenization 手動の均質化
homogenization with a tissue grinder 組織グラインダーによる均質化
Potter-Elvehjem homogenizer ポッター-エルフェージェムホモジナイザー
Potter‐Elvehjem homogenizer ポッター‐エルベージェムホモジナイザー
The tissue is homogenized in a 0.25M sucrose solution containing 1.0 mM EDTA and 0.1 mM PMSF using a Potter-Elvehjem homogenizer with a teflon pestle. この組織を、テフロン製の乳棒を備えたポッター-エルヴェジェム(Potter-Elvehjem)ホモジェナイザーを用いて、1.0mM EDTAおよび0.1mM PMSFを含有する0.25Mショ糖溶液中に均質化する。



Definition:
http://en.wikipedia.org/wiki/Homogenization
Cell biology
Homogenization is a process that involves breaking apart cells - releasing organelles and cytoplasm. When the purpose is to extract organelles, it is frequently done in two steps; first using a blender to break the tissue up, and then with an ultrasonic or mechanical tissue disruptor. The organelles are then generally separated using differential centrifugation. When the aim is to extract nucleic acids, the tissue is often ground in a mortar and pestle under liquid nitrogen.
Sometimes, however, a much milder procedure is followed, where the aim is to collect whole, intact cells. Homogenized cells must be kept at low temperatures to prevent autolysis and kept in an isotonic solution to prevent damage by osmosis.

Di Cola D, Federici G Biochem J. 1982 Aug 1; 205(2):265-269 1 ...
Effect of NADPH at increasing concentration of GSH Tissue homogenization and ... pellet with the aid of two or three strokes of a Potter homogenizer. ...
www.pubmedcentral.gov/articlerender.fcgi?artid=1158477 - Similar pages
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