Glossary entry (derived from question below)
German term or phrase:
über 0,2 µm sterilfiltrieren
English translation:
filter (the solution) through a 0.2 µm filter under sterile conditions
Added to glossary by
Rowan Morrell
Apr 28, 2007 11:26
17 yrs ago
German term
New Zealand
Committed to the Customer
Native in: English (Variant: New Zealand) 
Works in: French to English, German to English, Spanish to English
über 0,2 µm sterilfiltrieren
German to English
Science
Chemistry; Chem Sci/Eng
Raw Material SOP
"Herstellung einer 2 M Lösung Tricin pH 8,9 (Ammoniak)
Zur Chargenbewertung werden 50 ml Lösung angesetzt.
M = 179,2 g/mol
17,92 g Tricin in ca. 25 ml Reinstwasser lösen und unter Rühren mit konz. Ammoniak auf pH 8,9 bei RT einstellen. Tricin geht erst durch Zugabe von Ammoniak und durch leichtes Erwärmen in Lösung. Nach eingestelltem pH Lösung auf 50 ml mit Reinstwasser auffüllen, ***über 0,2 µm sterilfiltrieren*** und in ein Polypropylen Gefäß abfüllen."
From an SOP for handling various pharmaceutical raw materials. I don't understand the phrase "über 0,2 µm sterilfiltrieren". Filter through 0.2 µm of what? And how do I fit the sterile bit in there? "Sterilely filter" sounds VERY clumsy. TIA for your assistance.
Zur Chargenbewertung werden 50 ml Lösung angesetzt.
M = 179,2 g/mol
17,92 g Tricin in ca. 25 ml Reinstwasser lösen und unter Rühren mit konz. Ammoniak auf pH 8,9 bei RT einstellen. Tricin geht erst durch Zugabe von Ammoniak und durch leichtes Erwärmen in Lösung. Nach eingestelltem pH Lösung auf 50 ml mit Reinstwasser auffüllen, ***über 0,2 µm sterilfiltrieren*** und in ein Polypropylen Gefäß abfüllen."
From an SOP for handling various pharmaceutical raw materials. I don't understand the phrase "über 0,2 µm sterilfiltrieren". Filter through 0.2 µm of what? And how do I fit the sterile bit in there? "Sterilely filter" sounds VERY clumsy. TIA for your assistance.
Proposed translations
(English)
| 4 +5 | sterile filter the solution over a 0.2 µm filter |
Inge Grueneberg
|
Proposed translations
+5
19 mins
Inge Grueneberg
Germany
Germany
Native in German
Specializes in field
Germany
for top-quality translations
Native in: German
Works in: German to English, English to German
Related KudoZ points
:
16
Selected
sterile filter the solution over a 0.2 µm filter
meant is the process of filtering through a pore size of 0.2 µm, which largely eliminates bacteria, since they are usually larger in size.
The filtering assembly is variable. For small volumes you may use a syringe onto which one can fix a disposable syringe filter. Or you have a complete presteriled filter device with funnel, filter (0.2 µm) and container.
Anyway you always sterile filter your solution over a 0.2 µm pore size filter, actually one may leave out the last part and just say sterile filter the solution, however since it is an SOP...
Actually the instruction is incorrect. If you sterile filter first and then transfer the soultion to another vessel it becomes unsterile again. One should sterile filter the solution into a sterile PP Flask...
If that aint enough!
The filtering assembly is variable. For small volumes you may use a syringe onto which one can fix a disposable syringe filter. Or you have a complete presteriled filter device with funnel, filter (0.2 µm) and container.
Anyway you always sterile filter your solution over a 0.2 µm pore size filter, actually one may leave out the last part and just say sterile filter the solution, however since it is an SOP...
Actually the instruction is incorrect. If you sterile filter first and then transfer the soultion to another vessel it becomes unsterile again. One should sterile filter the solution into a sterile PP Flask...
If that aint enough!
4 KudoZ points awarded for this answer.
Comment: "I rather prefer Gillian's phrasing, so this is a de facto 4 points to her! But thanks to everyone who contributed here - appreciate your assistance. Apologies for the somewhat slow grading."
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